Validation of a rapid, saliva-based, and ultra-sensitive SARS-CoV-2 screening system for pandemic-scale infection surveillance

Without any realistic prospect of comprehensive global vaccine coverage and lasting immunity, control of pandemics such as COVID-19 will require implementation of large-scale, rapid identification and isolation of infectious individuals to limit further transmission. Here, we describe an automated, high-throughput integrated screening platform, incorporating saliva-based loop-mediated isothermal amplification (LAMP) technology, that is designed for population-scale sensitive detection of infectious carriers of SARS-CoV-2 RNA. Central to this surveillance system is the “Sentinel” testing instrument, which is capable of reporting results within 25 min of saliva sample collection with a throughput of up to 3840 results per hour. It incorporates continuous flow loading of samples at random intervals to cost-effectively adjust for fluctuations in testing demand. Independent validation of our saliva-based RT-LAMP technology on an automated LAMP instrument coined the “Sentinel”, found 98.7% sensitivity, 97.6% specificity, and 98% accuracy against a RT-PCR comparator assay, confirming its suitability for surveillance screening. This Sentinel surveillance system offers a feasible and scalable approach to complement vaccination, to curb the spread of COVID-19 variants, and control future pandemics to save lives.

www.nature.com/scientificreports/ of 4-52% 7 ). Since presymptomatic and asymptomatic individuals often vary considerably in the load of SARS-CoV-2 virus they carry, surveillance strategies should ideally have sufficient sensitivity to identify all potentially infectious individuals. This will be critical for identifying 'superspreaders' , who have been estimated to account for up to 40% of subsequent infections, despite not necessarily having particularly high viral loads in their bodily fluids (including saliva). 'Superspreaders' are instead postulated to spread the virus more rapidly by alternative means, such as increased aerosol production 8,9 . The combined presymptomatic and asymptomatic infection rate has been estimated to exceed 50% in some unvaccinated populations, meaning that testing only symptomatic individuals will fail to detect many SARS-CoV-2 diagnoses. In the UK, 70% of cases were reported as undetected (8), while a French study found that 93% of infected persons were undetected in a single test 1,5 . A comprehensive and detailed study from the Vo municipality in Italy, where 80% of the population was tested twice for the virus, found that 42% of those infected did not show any symptoms 10 . A subsequent report revealed that as much as 59% of all SARS-CoV-2 viral transmission came from asymptomatic transmission events (comprising 35% from presymptomatic individuals and 24% from individuals who never went on to develop COVID-19 symptoms) 11 .
While vaccination greatly reduces the degree of asymptomatic virus carriage, a study showed that viral RNA from SARS-CoV-2 'delta' variant breakthrough infection cases (79% of whom were asymptomatic) could still be detected in vaccinated individuals for up to 33 days (median 21 days) from their original diagnosis 12 . This study found that delta viral loads were 251 times higher than viral loads reported from infections of previous SARS-CoV-2 strains detected almost a year earlier from the same region using equivalent RT-PCR tests 12 . The increased infectiousness of the delta variant may ultimately be due to these much higher peak viral loads, which were shown to peak within 2-3 days on either side of the time of symptom development. These findings suggest that a more comprehensive vigilance strategy may be required to rapidly detect infectious individuals in the community, irrespective of whether they have symptoms, and regardless of vaccination status, to effectively contain the spread of the more infectious delta and omicron variants into vulnerable populations or populations with low prevalence.
The main bottlenecks with current testing strategy are limited capacity scaling, route of the sampling and time that is required for testing. Current standard of testing involves RT-PCR analytics, that will take at least 2 h to run from purified RNA, in addition to time to take nasopharyngeal swabs and the limit of skilled personnel who can safely do so. After taking sample, RNA needs to be extracted and purified, followed by the setting up and running the reaction. Typical times from sampling to testing with RT-PCR are at least 6 h, or even longer in practice. Capacity limitations are caused by the number of molecular diagnostics machines available in testing laboratories and by the limited number of personnel that can collect and process the samples. Cumulative delays in the sampling, testing, and reporting the test results cause the escape of infectious individuals from surveillance. Therefore, the overall testing speed could be considered the biggest challenge with current pandemic.
To address sampling bottlenecks, we developed a saliva-based testing system that is robust, sensitive and easy to perform from multiple parallel self-sampling stations. The testing procedure begins with automated self-administered barcode ID scanning and saliva sampling via a small swab in less than 30 s per scanning point. Saliva sample tubes can then be continuously loaded onto a conveyor reflow oven for heated inactivation within 10 min, before parallel automatic tube decapping and transfer of samples into a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay incubation for 20-25 min.
We developed an automated random access, continuous flow robotic system known as the Sentinel for scaling the entire process of sample dispensing, incubation, and detection of RT-LAMP reactions while securely reporting results in as little as 35 min including sample preparation. The scalable screening capability is intended to be rapidly deployed at low cost for regular surveillance testing of large numbers of individuals (particularly at borders, ports, and public sporting and entertainment venues). This surveillance system is also intended to enable rapid containment of emerging SARS-CoV-2 variants (omicron) that might eventually escape vaccine protection.

Results
Here, we report a proof-of-concept pilot for the integration of all the required components of such a testing system, incorporating saliva sample preparation, optimized reverse transcriptase-loop mediated isothermal amplification (RT-LAMP) chemistry and the development of a random access, continuous flow system for scaling the entire process of sample dispensing, incubation, and detection of reactions while securely reporting results in real time.
We chose to employ RT-LAMP chemistry for rapid virus detection, since its sensitivity is much closer to RT-PCR than to lateral flow assay (LFA)/lateral flow device (LFD) or rapid antigen tests (RAT) 13,14 and because RT-LAMP enzymes are relatively tolerant of inhibitors present in saliva, allowing readout of accurate test results within minutes of direct sample collection 15 . Identifying robust and sensitive RT-LAMP chemistries for surveillance screening. We first performed a side-by-side comparison of RT-PCR against a range of fluorometric RT-LAMP chemistries utilising five published primer sets and using RNA extracted from 20 clinical saliva samples obtained from individuals who had received a positive nasopharyngeal swab RT-PCR test result, including 10 samples with Ct above 34 indicating very low viral loads. For this evaluation, we selected the most sensitive RT-PCR assay in the FDA's published list of 117 SARS-CoV-2 tests (limit of detection (LoD): 180 viral copies/ml) as our benchmark comparator assay 16 .
When the same amount of RNA was added to each assay to allow a fair and direct comparison, RT-LAMP detected SARS-CoV-2 virus in 10 out of 20 samples versus 11 out of 20 for RT-PCR all these samples with Ct at least 33 or less. This result indicates that RT-LAMP has comparable performance to RT-PCR with the same  www.nature.com/scientificreports/ Assessing the performance of direct RT-LAMP for surveillance screening. Using this optimized sample inactivation method, we next compared various dual read-out (fluorometric and colorimetric) direct RT-LAMP chemistries with two approved RT-PCR diagnostic tests, each requiring RNA to be extracted, and with two direct RT-PCR tests, on the same 20 clinical saliva samples (Tables 2, 3). Direct RT-LAMP detected virus in 15 out of 20 samples, compared to 17 and 18 out of 20 for the two RT-PCR diagnostics assays ( Table 2). The best RT-LAMP assays could detect viruses in all samples corresponding to a Ct of 33/34 and below, where Ct values were derived from an approved diagnostic comparator test (Table 3). Encouragingly, detection of samples with low viral loads (Ct > 40) was also sometimes observed, albeit with less than 100% sensitivity. The performance of direct RT-LAMP was comparable to that of direct RT-PCR, with direct RT-LAMP detecting virus in 15 out of 20 samples, versus 14 or 16 (depending on the PCR kit used) out of a total of 20 with direct RT-PCR ( Table 2). The detection limit to achieve 100% sensitivity of direct RT-LAMP versus direct RT-PCR corresponded to a Ct of 40 and below (Table 3). Both primer sets targeting the N and E genes (in particular, the CE-marked Hayat Genetics diagnostic kit) performed better than the ORF1ab-targeted Huang O117 primer set 25 . Time-to-threshold (TTT) values for all RT-LAMP assays performed were between 8.5 and 26.5 min, with a mean TTT of 15.1 min. Taken together, these data show that our direct RT-LAMP method (using optimized saliva inactivation and Hayat Genetics chemistry) is rapid and sufficiently sensitive to detect infectious levels of SARS-CoV-2 virus directly from as little as 1.25 μl of saliva diluted in AviSal buffer. Inconsistent detection of samples with very low viral loads would not be expected to be relevant to COVID-19 containment measures, since those samples likely correspond to individuals thought not to be contagious 26 . If we consider only those positive samples with Ct values below 33 (green line in Table 2) from potentially infectious individuals, then there is 100% concordance between RT-PCR tests and RT-LAMP targeting the N and E genes.
Our assay validation studies demonstrated that a variety of RT-LAMP chemistries can be used to detect the SARS-CoV-2 virus directly from as little as 1.25 μl of saliva input diluted in AviSal buffer with high sensitivity across samples spanning a wide range of viral loads (Ct values ranging from 19 to > 40) when compared with RT-PCR "gold standard" comparator assays for the detection of SARS-CoV-2 (Tables 2, 3). Importantly, using the optimized RT-LAMP assay system, we observed 100% sensitivity in detecting SARS-CoV-2 across all specimens with potentially contagious viral loads, corresponding to Ct values up to 33 in accredited diagnostic RT-PCR tests. These data suggest that our surveillance system is likely to be sufficiently sensitive to detect all infectious SARS-CoV-2 carriers, given that an individual's viral load beneath our limit of detection is generally thought not to be contagious, given multiple reported failures to cultivate any SARS-CoV-2 virus using in vitro cell culture from samples with Ct values of over minimum reported thresholds ranging from 24 to 33 [26][27][28] . Indeed, sequencing of SARS-CoV-2 transcripts from infected cells recently established that full genome sequence coverage was not observed in samples with Ct values greater than 32 (measured with the same diagnostic Perkin Elmer RT-PCR assay which we used here as our comparator 29 ), suggesting that any RT-PCR detection of small SARS-CoV-2 genomic fragments in samples beyond this threshold would not likely reflect intact viable virus with potential to be infectious.
Optimising RT-LAMP specificity to minimise false positive reactions. Sequencing of products of LAMP reactions has demonstrated that this amplification chemistry can allow development of false positives in the absence of template due to nonspecific primer-dependent amplification. These nonspecific reaction products, the prevalence of which varies between primer sets, normally emerge later in the reaction incubation 30 , explaining why the 'time-to-positive' relates to specificity as well as to viral load.
Maximum incubation times are therefore typically specified in RT-LAMP protocols to minimise the chance of such false positives arising, even at the expense of reduced sensitivity. We found that in the absence of a sealed heated lid on the Sentinel surveillance instrument, a high rate of evaporation during a standard 30-min RT-LAMP incubation at 65 °C resulted in the early emergence of false positives after approximately 20 min, causing the reaction specificity to drop below 100%. This finding is particularly relevant to screening on the Sentinel surveillance instrument (see below), which lacks a heated lid seal to minimise evaporation. To better understand the correlation between the rate of false positive production and evaporation, we ran multiple negative control colorimetric RT-LAMP reactions in replicate, with both NEB (M1804 plus E1/N2 primers) and Hayat Genetics chemistries using saliva samples negative for SARS-CoV-2 and extended the reaction run time beyond the standard 30-90 min (Fig. 1). In the absence of mineral oil, 10% of NEB reactions were positive by 30 min, with 100% becoming positive by 40 min. The more specific Hayat Genetics assay chemistry was slower to produce false positives, with all reactions still negative at 40 min but eventually turning positive by 70 min in the absence of mineral oil. These data confirm that RT-LAMP is prone to false positives and that choice of optimal chemistry and preventing evaporation is critical in ensuring high specificity of RT-LAMP by 30 min. The Hayat Genetics assay also exhibited superior specificity. However, to further mitigate the effect of evaporation, regardless of the chemistry used, we incorporated an oil overlay barrier in all RT-LAMP reactions.
Confirmation of the detection of "Delta" and "Omicron" variants by RT-LAMP. We next investigated whether the most effective of the RT-LAMP chemistries we tested (Hayat Genetics) could detect RNA from the SARS-CoV-2 "delta" (B.1.617) and "omicron" (B.1.1.529) variants. As expected, given that the Hayat RT-LAMP primer set amplifies genomic regions that are conserved across variants, we could robustly detect synthetic RNA from the delta and omicron variants across a wide range of concentrations within 20 min (Suppl. Figure S2A,B).
Designing an RT-LAMP surveillance system supporting continuous loading of samples at random. We designed an automated RT-LAMP surveillance system, specifically for ultra-high-throughput www.nature.com/scientificreports/  Target T1  T2  T3  Lowest  10  22  23  21  21 13.5 12.5 11.5 9.  - www.nature.com/scientificreports/ detection of viral nucleic acids, directly from saliva samples taken on a population-wide scale (Fig. 2). This integrated system combines an optimized system for efficient sample collection preparation in barcoded tubes, which are rapidly heat inactivated and consolidated into racks for automated uncapping and continuous loading onto a robotic instrument that automatically dispenses samples and reagents and continuously scans and reports results.
The integrated system was designed to address key bottlenecks identified in existing surveillance technologies by incorporating the following features: 1 A scalable method for safe collection, heat inactivation and tracking of saliva samples. 2 Optimized sample processing and LAMP assay choice to maximise sensitivity/specificity. 3 Incorporation of a continuous flow, random access loading of racks of sample tubes/plates onto the system with integrated sample tracking and reporting to support ultra-high throughput applications.
The capacity of conventional molecular diagnostic instruments is constrained by largely batch-based sample loading logistics, frequently resulting in rate-limiting queuing of microplates awaiting access to the instrument. This batch constraint also applies to large 'continuous flow' diagnostic instruments 31 , which sometimes include multiple incubation stations employed on a range of predetermined schedules to achieve faster cycle times, which can be less time-and cost-efficient, particularly at lower throughputs, while samples are accumulated to achieve optimal capacity.
To address this scalability bottleneck, the 'Sentinel' LAMP instrument (Fig. 2B) was designed as a truly continuous flow system, treating the arrival and disposal of each plate into the system completely independently. The Sentinel instrument can perform RT-LAMP tests at up to 3840 tests per hour, with potential for further increases in scale in the future, by switching from 96-to a 384-well format.
The Sentinel system was conceived to incorporate a resource scheduling and monitoring system tasked with processing as many microplate assays as possible within a given period, balancing upstream processing of samples with downstream reaction and disposal steps, to efficiently schedule the arrival and departure of microplates being analysed into selected incubation slots within a common isothermal incubation zone.

Sample stability experiment: Hayat RT-LAMP assays (N & E targets).
To establish the feasibility of saliva sample collection without requiring cold chain logistics, we tested how our sample collection procedure could affect the outcome of RT-LAMP assays and challenged the robustness of testing with different storage conditions using AviSal saliva collection buffer. This study established that collected saliva samples spanning a wide range of viral loads are stable in storage media without loss of sensitivity during preinactivation storage at RT for up to 48 h, post inactivation at RT for 2 h or 4 °C for 48 h, and through a freeze/thaw cycle (Fig. 3).
Colorimetric LAMP limit of detection on the clinically deployed Sentinel instrument. Next, we conducted a study to pilot the implementation of the Sentinel Surveillance instrument in the context of a clinical microbiology service laboratory in San Sebastian, Spain. Saliva samples from individuals who had tested positive by an approved diagnostic RT-PCR swab test were frozen prior to testing on the Sentinel Instrument. Six of these saliva samples, with distinct viral loads, were serially diluted to obtain a panel of 36 samples spanning a wide range of Ct values. These diluted samples were further diluted in a commercially available saliva transport medium-Saliva Transport buffer M (Vitro SA, Spain)-and heat inactivated for 10 min at 95 °C. Direct RT-PCR (Seegene Allplex) was performed on 5 μl of these heat-treated saliva dilutions.
The NEB colorimetric RT-LAMP (N&E-gene, M1800 2 × LAMP mix) was run on the Sentinel using 3 μl of the same template as RT-PCR. Table 4A shows the results of RT-PCR and RT-LAMP for all 36 saliva samples organized by sample, while Table 4B shows the same data organized by Ct value. Table B clearly demonstrates that all saliva samples up to a Ct of 31.1 were detectable by RT-LAMP. Higher Ct values could also be detected, but with inconsistent reliability, indicating that the limit of detection for VTM/heat-treated saliva samples combined with NEB M1800/N&E chemistry corresponds to a Ct of 31.1, which represents a slightly less sensitive detection threshold than we obtained for similar chemistry with an alternative AviSal Sample Transport Buffer (Tables 2, 3). This decreased sensitivity observed may be due to differences in the composition of the alternative sample collection buffer used or, alternatively, could result from differences in sensitivity between colorimetric/ fluorometric chemistries or differences in the comparator RT-PCR assays used in each case. Nevertheless, this pilot implementation study demonstrated that the SARS-CoV-2 RT-LAMP assay is sufficiently versatile to be adapted to be compatible with local sample collection processes of a routine clinical diagnostics service.  Table 5). The efficiency (accuracy) of the test was 98%. Changing the maximum Time to threshold (TTT) cutoff time altered the sensitivity and the specificity of the test (Table 5). Shorter cut-off times (20 min) resulted in better specificity (99.6%) but a lower sensitivity (95.3%). TTT values were in a perfect correlation with the Ct values of the RT-PCR results showing (Suppl. Figure S3; Suppl. Table S1). A comparative analysis of Hayat Genetics and Optigene Direct RT-LAMP tests was performed on the NIBSC (National Institute for Biological Standards and Control, UK) standardised SARS-Cov-2 virus panel supported these findings (supplementary figure S3). Each assay was carried out according to their recommended instructions for use and run on the same Sentinel instrument. Hayat Genetics/Avicena test was able to detect the standards to the maximum Ct in the NIBSC standards panel of (Ct = 29), whereas the Optigene assay could only reliably detect those standards up to a higher viral load threshold (Ct of 21). A limit of detection (LoD) assay of Hayat LAMP was also determined using a 10-fold dilution series of WHO International Standard for SARS-CoV-2, which was further diluted 1 in 5 in Avisal, and found to be at most 1500 copies/ml (30 copies per 20 μl reaction (supplementary figure S3). Analytical specificity of the Hayat Genetics chemistry was also assessed using a panel of analytical microbial standards, including 18 respiratory pathogens (RCPAQAP Molecular Respiratory Pathogens 2020), none of which were detected in any of the 4 replicates of the standards run in the LAMP assay (Suppl . Table S1).
We also compared the performance of Avicena RT-LAMP test to other approved testing solutions, direct RT-LAMP and RAT/LFA/LFD test. The Hayat RT-LAMP assay run on an Avicena Sentinel instrument (designated as Hayat/Avicena test) performed better that direct RT-LAMP (Optigene) run on the recommended Genie instrument or on the Sentinel instrument (Fig. 4). The Hayat/Avicena test worked much better that LFA/LFD/ RAT test run with the recommended saliva protocol (Fig. 4). The RAT test performed more poorly with only 50% of detection even of high viral load samples.

Discussion
Addressing the need for more sensitive rapid surveillance of asymptomatic individuals. While the diagnostic gold standard RT-PCR test is generally sufficiently sensitive to detect all infected individuals, this technology lacks sufficient speed (taking hours) and throughput (at most a few thousand samples processed per instrument per day) to support routine surveillance screening and quarantine applications. In addition, nasopharyngeal sampling requires trained personnel, is inconvenient, and has decreased participant acceptance if multiple testings within a short period are required. Alternatively, more acceptable rapid tests are emerging in response to these challenges but are not all sufficiently sensitive to reliably prevent outbreaks, particularly as more infectious variants continue to arise. A recent systematic review suggested an average analytical sensitivity overall for lateral flow antigen (LFA) tests of 72% 32-34 , while a systematic review of 58 LFA test evaluations found that their sensitivity was reliable only when detecting samples with high (i.e. Ct < 25) viral loads. This explains why their sensitivity was significantly lower in asymptomatic people, where LFA sensitivity ranged from 28 to 69% [32][33][34] . Similarly, a large study of 2215 people attending a diagnostic centre showed the sensitivity of the Roche and Abbott LFA tests to be only 60.4% and 56.8%, respectively, in detecting RT-PCR-positive (Ct < 30) individuals 35 , which could still be infectious as outlined above. Real-world experience has established that a significant proportion of international air travellers who test positive for SARS-CoV-2 upon arrival are asymptomatic 36,37 and would not be reliably detected by lateral flow tests.
Therefore, surveillance testing strategies based solely on such insufficiently sensitive LFA tests may be counterproductive by providing a false sense of security. This was highlighted in an outbreak in Liverpool UK, where 60% of SARS-CoV-2 infections (33% of which had high viral loads) were not detected using LFA 38 . A study from the University of Birmingham in April 2021 found that only 5% of SARS-CoV-2-infected individuals were detected by LFA with potentially infectious (Ct < 33) viral loads 39 . In comparison, the test we are reporting here has 98.7% of sensitivity, 97.6% of specificity, and 98% of accuracy/efficiency at this range of viral loads. The recent  www.nature.com/scientificreports/ experience of an outbreak at the Tokyo Olympics, traced to an individual who had tested negative in an LFA test 40 , has further highlighted the risks of using LFA tests for asymptomatic surveillance of vulnerable groups. Rapid SARS-CoV-2 nucleic acid test (NAT) technology, such as the Abbott ID diagnostic test, has therefore been advocated as an alternative or adjunct to LFA tests for COVID-19 surveillance applications, particularly in the next phase of the pandemic 41 . However, a systematic review of pooled data from the multiple clinical evaluations of the Abbott ID Now test found that its sensitivity was inferior to comparator RT-LAMP tests on crude samples 42 .
We have prototyped an RT-LAMP-based screening system, that combines sufficient sensitivity for effective viral surveillance with feasible scalability to very high throughput. Given that the viral load in samples from infected but presymptomatic or asymptomatic people can take several days to reach the limit of detection of even the most sensitive tests (such as RT-PCR and RT-LAMP), frequent testing is required to maintain effective viral surveillance 43 . This is especially important given evidence of SARS-CoV-2 transmission from asymptomatic people with low viral loads, which can gradually increase over several days 44 , even in vaccinated people 12 , suggesting that optimal surveillance testing should therefore involve sequential NAT tests.
A recent report of screening 86,000 such samples, with an analytical sensitivity of 87.61% and a specificity of 100% of RT-LAMP in detecting potentially infectious viral loads (Ct < 33) from directly screening saliva clearly showed the value of RT-LAMP for infection surveillance of asymptomatic individuals, if it could be conducted routinely 45 .
Therefore, we chose to employ an automated RT-LAMP-based screening system in combination with the optimised upstream saliva processing protocol. This approach provides sufficient sensitivity for effective viral Table 5. Hayat genetics RT-LAMP sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and efficiency (A). Four different TTT cut-off times were used for comparison with RT-PCR results. Sensitivity and specificity with 95% confidence limits (B). www.nature.com/scientificreports/ surveillance with feasible scalability to ultra-high throughput screening. Saliva sampling, as proposed here, makes repeated testing quicker without compromising testing quality. Using saliva as a test substance is fundamental for the efficient large scale testing strategy and makes frequent testing more-feasible. For example, weekly surveillance testing using saliva-based RT-LAMP has recently been advocated as a feasible means of complementing vaccines to contain the spread of highly infectious pandemic agents such as SARS-CoV-2 46 . Travel biosecurity could therefore be enhanced by sequential testing of the passengers both before departure and upon arrival using rapid NAT tests such as RT-LAMP. Using saliva samples instead of nasopharyngeal collection, makes this approach practical and reliable.
The integrated high-throughput surveillance system described above allows rapid turnaround from convenient saliva sampling combined with highly efficient analysis, which may help facilitate community acceptance of repeated testing. Importantly, the confidentiality of data from tested individuals is also maintained within the Sentinel sample tracking system, based on anonymous digital tokens, ensuring that the instrument's reporting of test results remains deidentified. Test results reported from the instrument can only be associated with participant identities externally by authorised entities, such as participants themselves or accredited pathology services, who have exclusive access to the required information.
Given limited opportunity to test our system in the field locally (since due to strict border controls, Western Australia experienced no cases of community transmission of SARS-CoV-2 between April 2020 and October 2021), we collaborated with international COVID screening laboratories for the purposes of this study. Extended large-scale deployments of this system will allow further optimization of testing procedures, adapted to specific feedback from different testing environments. Differences in the operations of seaports, airports and sporting venues or ships will therefore likely necessitate refinement to maximise the process efficiency and acceptability. For example, we are now collaborating with resources companies requiring high throughput rapid biosecurity screening measures, to protect their remotely deployed workforce from risks to occupational health and safety imposed by severe infection.
Summary. The Sentinel surveillance system combines the required components of a sensitive molecular screening system to support efficient detection of all potentially infectious asymptomatic carriers at pandemicscale. Within the same platform we combined highly parallel sample processing and sensitive biochemistry with the development of random access, continuous flow robotic analysis system.
We have established proof-of-concept for integration of a saliva-based RT-LAMP assay with a surveillance instrument, currently capable of screening up to 4000 reactions per hour with up to 98% of accuracy. This versatile pathogen vigilance platform offers a unique combination of accuracy and scalability to provide a feasible way to mitigate the risk of viral transmission as borders open and new viral threats arise in the future.

Methods
Sample preparation. The samples used in this study were collected from the Sir Charles Gairdner and Osborne Park Health Care Group. All methods were carried out in accordance with relevant guidelines and regulations. The study protocol was approved and endorsed by the Sir Charles Gairdner and Osborne Park Health Care Group Human Research Ethics Committee. Saliva samples were collected, with informed consent, from returned travellers in hotel-quarantine who had been diagnosed as SARS-CoV-2 positive from an approved RT-qPCR diagnostic test (using nasopharyngeal swabs) by the Western Australian state pathology service laboratory (PathWest). None of the individuals required hospitalization. Samples were diluted in an equal volume of viral transport medium (VTM) and stored at − 20 °C. Saliva/VTM was then diluted in AviSal Sample Collection Buffer (Hayat Genetics) and heat inactivated at 95 °C for 10 min before being added to Direct RT-qPCR and RT-LAMP reactions.

Reverse-transcription loop-mediated isothermal amplification (RT-LAMP). RT-LAMP primer
sequences used in this study have been published elsewhere and are as follows: Zhang E1/N2 (16); Huang O117 and Huang S17 (both Huang primer sets described in in the main text (25)). For internal control reactions, we used rActin primers from NEB.
Oligonucleotides were synthesized by Integrated DNA Technologies, Inc. (IDT, Coralville, IA, USA). F3, B3, LoopF and LoopB primers were desalted after synthesis; FIP and BIP primers were HPLC-purified. 10 × RT-LAMP working primer mixes were prepared in nuclease-free water and contained 2 μM F3, 2 μM B3, 16  www.nature.com/scientificreports/ reached a threshold, set as 1.5 × baseline fluorescence signal intensity-corresponding to more than 3 standard deviations from the mean baseline signal). A time-to-threshold was calculated for each reaction. Colour change was confirmed by photography with a smartphone at the beginning and end (30 min) of the reaction and blindcoded. Incubation on ice for 1-2 min improved colour contrast, making scoring easier. A positive result was scored when colour had changed from pink to orange or yellow.
Independent comparator study. The samples used in this study were collected as part of an asymptomatic screening programme. Sample information was collected using an app, which blinded the source of the samples to the lab. A study was performed using these blinded samples to determine the specificity and sensitivity of the RT-LAMP test by comparing its results to an approved RT-PCR diagnostic test. The comparator study used 150 genuine PCR-positive samples with widely ranging viral load and 250 genuine PCR-negative samples. All samples were analysed by RT-qPCR using ELITE MGB Kit used on an Elite InGenius system and results were compared head-to-head with Hayat Genetics LAMP results run on a Sentinel instrument. Different analytical cut-off times for detection Time-To-Threshold values (TTT) were assessed to determine the effect on sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and efficiency of the assay. The Hayat/Sentinel test was also compared to a direct saliva LAMP assay used in the UK developed by Optigene Ltd run on the recommended Genie instrument and to an authorized saliva protocol for a RAT approved for use within the UK (SureScreen Diagnostics).

Data availability
All data are available in the main text or the supplementary materials.